"""Plot physical chemostat equilibria; save basename PNG to the CWD. Tested with Python 3.14.4, Matplotlib 3.10.7, NumPy 2.3.5. Run from _media: MPLCONFIGDIR=/tmp/chemostat-mpl python3 ../chemostat-washout-equilibria.py The algebraic positive branch is plotted only where biomass is nonnegative. At D=0 the positive-branch limit is shown open: the flow-free model is different. """ from pathlib import Path import matplotlib matplotlib.use("Agg") import matplotlib.pyplot as plt import numpy as np def main(): mu_max, k_s, feed, yield_coefficient = 1.0, 1.0, 9.0, 0.5 critical = mu_max * feed / (k_s + feed) dilution = np.linspace(0.0, critical, 451) nutrient = dilution * k_s / (mu_max - dilution) biomass = yield_coefficient * (feed - nutrient) plt.rcParams.update({"font.size": 11, "axes.spines.top": False, "axes.spines.right": False}) fig, axes = plt.subplots(1, 2, figsize=(10.4, 4.8), dpi=100, layout="constrained") fig.patch.set_facecolor("white") for ax, branch, washout, title, ylabel in zip( axes, (nutrient, biomass), (feed, 0.0), ("Residual nutrient", "Biomass in the vessel"), (r"$s_*$ (g nutrient/L)", r"$x_*$ (g dry biomass/L)")): ax.set_facecolor("white") ax.plot(dilution[1:], branch[1:], color="#17679b", lw=2.5, label="Stable positive culture") ax.scatter([0], [branch[0]], s=38, facecolors="white", edgecolors="#17679b", zorder=6) ax.plot([0, critical], [washout, washout], color="#c56a16", ls="--", lw=2, label="Unstable washout") ax.plot([critical, 1.15], [washout, washout], color="#c56a16", lw=2.5, label="Stable washout") ax.axvline(critical, color="#777777", ls=":", lw=1.5) ax.scatter([critical], [washout], color="#444444", s=42, zorder=7) example = k_s * 0.5 / (mu_max - 0.5) if ax is axes[1]: example = yield_coefficient * (feed - example) ax.scatter([0.5], [example], color="#17679b", s=42, zorder=7) ax.annotate(f"D = 0.5: {example:g} g/L", (0.5, example), xytext=(0.12, 2.7 if ax is axes[0] else 2.2), arrowprops={"arrowstyle": "->", "color": "#17679b"}) ax.set(xlim=(-0.025, 1.15), xlabel=r"Dilution $D$ (h$^{-1}$)", ylabel=ylabel, title=title) ax.set_xticks([0, 0.3, 0.5, 0.7, 0.9, 1.1]) ax.grid(alpha=0.18) axes[0].set_ylim(-0.25, 10.0) axes[1].set_ylim(-0.15, 5.0) axes[0].legend(loc="upper left", fontsize=9, framealpha=1, facecolor="white") fig.suptitle("Chemostat washout at D = 0.9 h⁻¹\n" "μmax = 1 h⁻¹, Ks = 1 g/L, feed = 9 g/L, yield = 0.5 g/g") destination = Path.cwd() / (Path(__file__).stem + ".png") fig.savefig(destination, facecolor="white", transparent=False) plt.close(fig) print(destination) if __name__ == "__main__": main()