= A. J. Padgett-McCue
{c}
A. J. Padgett-McCue coauthored a 2000 study identifying a <chondroitin synthase> and establishing what <polysaccharide> it made. The work connected an <enzyme> with the alternating <glucuronic acid> and <N-acetylgalactosamine> units of <chondroitin>. The characterized <protein> carried both sugar-transfer activities required to extend this repeating chain.<Identification and molecular cloning of a chondroitin synthase from Pasteurella multocida type F by P. L. DeAngelis and A. J. Padgett-McCue>[Abstract, Journal of Biological Chemistry 275 (2000), pp. 24124–24129; DOI 10.1074/jbc.M003385200.]{ref}
The experimental reasoning combined <nucleotide sugar> specificity, <size-exclusion chromatography> and selective enzymatic digestion of the product. This gives a useful example of complementary measurements: a large molecular size establishes that a <polymer> was made, but does not identify its repeating chemical structure. Agreement between the sugar composition and the response to a <chondroitin>-cleaving <enzyme> supplied more specific evidence. The contribution is therefore a chemically tested biosynthetic assignment, rather than a prediction based only on similarity between <protein> sequences.<Identification and molecular cloning of a chondroitin synthase from Pasteurella multocida type F by P. L. DeAngelis and A. J. Padgett-McCue>[Abstract: donor specificity, gel filtration and contrasting lyase susceptibility.]{ref}
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