= A simple and very efficient method for generating cDNA libraries by U. Gubler and B. J. Hoffman
{tag=Work by U. Gubler}
{tag=Work by B. J. Hoffman}
{title2=1983}
https://pubmed.ncbi.nlm.nih.gov/6198242/
U. Gubler and B. J. Hoffman, Gene 25(2–3), 263–269, November 1983. DOI 10.1016/0378-1119(83)90230-5. The abstract describes first-strand synthesis followed by RNase H/DNA-polymerase-I second-strand synthesis, cloning from submicrogram mRNA amounts, and sequencing of a known bovine preproenkephalin transcript to test full-length fidelity.
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