= B. L. Seong
{c}
\b[B. L. Seong] is the author credited with <U. L. RajBhandary> in a 1987 study of structural recognition of <initiator transfer RNA>. They tested how a conserved <anticodon>-stem pattern distinguishes an <initiator transfer RNA> from an <elongator transfer RNA>.<Escherichia coli formylmethionine tRNA: mutations in GGGCCC sequence conserved in anticodon stem of initiator tRNAs affect initiation of protein synthesis and conformation of anticodon loop by B. L. Seong and U. L. RajBhandary>[Byline and Abstract, conserved stem pairs and initiation defects]{ref}
Separate assays compared charging, formylation, <bacterial initiation factor IF2> binding, <ribosomal P site> binding and <protein> synthesis. Greater accessibility of the <anticodon> loop to S1 nuclease connected the functional change with altered <RNA> conformation.<Escherichia coli formylmethionine tRNA: mutations in GGGCCC sequence conserved in anticodon stem of initiator tRNAs affect initiation of protein synthesis and conformation of anticodon loop by B. L. Seong and U. L. RajBhandary>[Abstract, biochemical characterization and S1-nuclease accessibility]{ref}
For an original assay example, suppose two preparations each contain 100 units of charged <transfer RNA>, and an earlier binding assay reports 80 units for each. If the later ribosomal readout reports 60 and 15 units, its fourfold difference cannot be explained by the amount of charged carrier alone. The example motivates measuring distinct steps rather than labeling every loss of activity a failure of binding. The numbers are illustrative; the paper's contribution is the experimentally supported distinction between factor recognition, ribosomal positioning and productive <biological translation>.
Back to article page
