= BD FACSCelesta Flow Cytometer User’s Guide by BD Biosciences
{c}
https://www.bdbiosciences.com/content/dam/bdb/marketing-documents/products-pdf-folder/instruments/research-cell-analyzers/bd-facscelesta-flow-cytometer-ruo-global.pdf
Manufacturer technical manual, Chapter 7, Technical overview: printed p. 102, About fluidics, describes laminar sheath flow and hydrodynamic focusing; p. 103 introduces optics. Printed p. 115, pulse processing: height is maximum digitized intensity; area integrates digitized samples over the acquisition window; width is separately measured. These definitions are instrument-specific implementations of pulse properties.
Back to article page