= Bisulfite sequencing
<Bisulfite sequencing> infers cytosine modification from differential chemical conversion before <DNA sequencing>. Under the relevant chemistry, unmodified cytosine is converted to uracil and is subsequently read as thymine, while 5-methylcytosine is retained as cytosine. Incomplete conversion, sequence coverage and strand assignment affect interpretation. Conventional <bisulfite sequencing> does not by itself separate 5-methylcytosine from every other conversion-resistant modification, so its signal requires a stated chemical scope. <A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands by M. Frommer and others>[Frommer and others, 1992, abstract: differential conversion and single-strand methylation display]{ref}
Back to article page
