= Bumetanide
<Bumetanide> is an organic <sulfonamide> compound used as a <loop diuretic>. Its neutral molecular formula is $\mathrm{C_{17}H_{20}N_2O_5S}$. It inhibits salt transport through <NKCC2>, the renal sodium–potassium–chloride cotransporter, making it useful both pharmacologically and as an experimental probe of <membrane transport>.<PubChem Compound database by National Center for Biotechnology Information>[Bumetanide, CID2471; chemical identity and loop-diuretic classification]{ref}
In a 1994 study, cloned kidney transporters were expressed in <Xenopus laevis> oocytes and distinguished by their transport behaviour and inhibitor sensitivity. This connected bumetanide-sensitive uptake to a specified transporter rather than to an unspecified effect of a crude tissue preparation.<Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney by G. Gamba and others>[Abstract; cloning, oocyte expression and transporter characterization]{ref}
The usual <NKCC2> transport cycle couples one sodium <ion>, one potassium <ion> and two chloride <ions>. Their transported charges sum to $+1+1-2=0$: a process can move substantial amounts of salt while remaining electrically neutral. An uptake measurement can therefore reveal inhibition even when net <electric current> alone would be an inadequate readout.
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