= Clement Howe
{c}
= Clement Peter Gregory Howe
{c}
{synonym}
= C. P. Howe
{c}
{synonym}
\b[Clement Howe] studied the melting and refolding dynamics of <RNA hairpins> and related <DNA> structures using <infrared spectroscopy>. His2023 University of York thesis identifies <N. T. Hunt> as supervisor and examines the influence of sequence and structure on these dynamics.<Sequence Dependent RNA UNCG and DNA TNCG Tetraloop Hairpin Melting and Refolding Dynamics Using Temperature-Jump Infrared Spectroscopy by Clement Howe>[Repository abstract and metadata]{ref}
Howe was the first author of the2022 study combining a rapid <temperature jump> with a thin sample's rapid cooling to observe both melting and refolding of a <UNCG tetraloop> RNA hairpin and its DNA analogue.<Measuring RNA UNCG Tetraloop Refolding Dynamics Using Temperature-Jump Drop Infrared Spectroscopy by C. P. Howe and others>[Title-page byline and Abstract]{ref}
A numerical comparison from that study separates forward and recovery dynamics. At70degrees Celsius, the reported melting times were $6.0\pm0.1$microseconds for the RNA hairpin and $0.8\pm0.1$microseconds for the DNA analogue, a ratio of about7.5. Their refolding occurred on similar timescales of roughly200microseconds. Similar recovery times therefore did not require similar melting times.<Measuring RNA UNCG Tetraloop Refolding Dynamics Using Temperature-Jump Drop Infrared Spectroscopy by C. P. Howe and others>[Abstract, UACG RNA and TACG DNA comparison]{ref}
The thesis extends the question by changing the stem sequence and using <two-dimensional infrared spectroscopy> to follow relationships between vibrational modes. Sequence changes can therefore be tested through both their spectral consequences and their time-dependent structural response.<Sequence Dependent RNA UNCG and DNA TNCG Tetraloop Hairpin Melting and Refolding Dynamics Using Temperature-Jump Infrared Spectroscopy by Clement Howe>[Abstract, chapters4–5]{ref}
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