Dounce homogenizer
= Dounce homogenizer
{c}
A <Dounce homogenizer> mechanically disrupts <cells> with a fitted <glass> pestle moving inside a <glass> vessel. Relative motion drives <fluid> through the narrow clearance, generating <deformation> and <shear stress> in the sample. The device is commonly operated manually. <Tissue Grinders by DWK Life Sciences>[DWK Life Sciences, Tissue Grinders: Dounce tube and fitted pestles]{ref}
Different pestle clearances produce different <flow> and disruption. The resulting preparation depends on sample mechanics and the applied motion, so homogenization is a controlled physical treatment rather than simple mixing. <Microscopy> can test whether the desired cellular material was released and which structures remained intact.