FITC (source code)
= FITC
{c}
= Fluorescein isothiocyanate
{synonym}
<FITC> is fluorescein isothiocyanate, a reactive derivative of <fluorescein> used to attach a fluorescent label to suitable targets. Its reactive group couples to accessible <amino groups>, so labeling a <protein> can make that protein observable by <fluorescence microscopy> or <flow cytometry>.
The label's presence is distinct from the specificity of the experiment. Unbound dye must be separated from the intended conjugate, and an unlabeled control estimates <autofluorescence>. Labeling can alter the target's behavior, while bleaching and environment-dependent emission affect measured <intensity>. https://documents.thermofisher.com/TFS-Assets/LSG/brochures/1601971-Antibody-Immunostaining-Guide.pdf[Thermo Fisher antibody immunostaining guide: fluorescein labeling and FITC reactive group]{ref}
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