Fluorescence emission spectrum (source code)

= Fluorescence emission spectrum
{title2=$F_{\rm em}(\lambda_{\rm em})$}

A <fluorescence emission spectrum> resolves emitted <photons> by <wavelength> at fixed excitation settings. Subtract a matched sample-container and solvent background, and correct the wavelength-dependent throughput and <photodetector> sensitivity when estimating the emitted distribution. A camera image through one <fluorescence emission filter> instead measures a weighted band <integral>, not a whole <fluorescence emission spectrum>. A <fluorescence lifetime> resolves delay after excitation; its horizontal axis is <time>, not <wavelength>.