= G. Gamba
{c}
<G. Gamba> is the first author of a 1994 study identifying two related salt-transport <proteins> in the mammalian <kidney>. The team cloned the corresponding <DNA> sequences and tested the expressed <proteins> in <Xenopus laevis> <oocytes>. One transported <sodium>, <potassium> and <chloride>; the other transported <sodium> and <chloride> without the same <potassium> requirement.<Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney by G. Gamba and others>[Abstract: cloned cDNAs and functional expression; Journal of Biological Chemistry 269, 17713–17722, 1994]{ref}
The transporters also differed in their responses to inhibitors, including <bumetanide>. Combining sequence comparison, transport assays and localization within the <kidney> established a family resemblance without assuming identical function. This is an important experimental distinction: a similar <protein primary structure> suggests a relationship, while controlled measurements of ion movement test what each <membrane transport> <protein> actually does.<Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney by G. Gamba and others>[Abstract: sequence similarity, differing ion requirements and inhibitor sensitivity, and intrarenal expression]{ref}
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