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Conservation genetics applies genetic evidence to maintaining biodiversity and viable biological populations. Measures of diversity, inbreeding and gene flow can identify isolation or help evaluate a proposed genetic rescue. Low diversity alone does not identify every ecological threat; demographic and habitat information remain necessary. In the Florida panther case, genetic and survival measurements together were used to assess restoration rather than treating introduced ancestry alone as proof of success. ^[ref]

Genetic rescue is an improvement in biological population fitness associated with introduction of genetic variation, often through gene flow into an inbred biological population. It differs from merely increasing numbers by adding individuals: the genetic contribution must improve descendant performance. The Florida panther restoration provided an empirical case in which introduced ancestry was followed by increased heterozygosity and improved survival measures. Outcomes depend on compatibility and ecological conditions, rather than making every introduction beneficial. ^[ref]

Warren E. Johnson led the 2010 assessment of genetic restoration in the Florida panther. The study combined population-genetic, demographic and biomedical observations after introducing Texas ancestry into the small Florida population. It reported increased heterozygosity, improved survival and fitness measures, and reduced correlates of inbreeding.^[ref]

The important scientific connection is between gene flow and population performance. Counting animals alone would not establish whether genetic diversity had changed; genotypes alone would not establish whether survival improved. Studying both helps evaluate genetic rescue, while the ecological setting and continued population management remain part of the interpretation.