Leica Microsystems, 1 October 2014. Definition and physical preparation sections: frozen fracture, optional vacuum sublimation, oblique metal coating and carbon support; SEM block-face versus TEM replica. Cited only for physical/material preparation; biological examples are outside this worker scope. Author profile www.leica-microsystems.com/science-lab/authors/detail/gisela-hoeflinger/ verifies the byline and coating expertise, with labeled portrait.