= Glucose clamp technique
{wiki}
<Glucose clamp technique: a method for quantifying insulin secretion and resistance. by R. A. DeFronzo and others> separates two questions. In a hyperglycemic clamp, variable <glucose> infusion holds <blood glucose> above baseline while sampled <insulin> reports the response. The 1979 article's abstract reports an early release burst followed by a slower rise. In a euglycemic <insulin> clamp, infused <insulin> is held elevated and variable <glucose> infusion holds <blood glucose> at baseline. Frequent blood sampling, a <glucose> analyzer, and controlled infusion apparatus make the imposed <concentration> a maintained condition rather than an uncontrolled consequence. <Glucose clamp technique: a method for quantifying insulin secretion and resistance. by R. A. DeFronzo and others>{ref}<Endotext/Assessing Insulin Sensitivity and Resistance in Humans>[pp. 4–6]{ref}
The maintained <glucose> level controls an important <confounder>: a change in <blood glucose> would itself change uptake and <hormone> release. At a <steady state>, <conservation of mass> gives uptake equal to infused supply plus endogenous production, after accounting for losses. Consequently, infusion alone estimates uptake only if endogenous production is negligible and the other balance assumptions hold. Labelled <glucose> measurements can estimate residual endogenous production; matching infused <insulin> across comparisons matters. Whole-body uptake cannot by itself identify the responding tissue, and plasma <insulin> reflects both release and removal. This interpretation uses the accessible 1979 abstract and the later authored methods chapter; the original article's full methods were not available for inspection.<Endotext/Assessing Insulin Sensitivity and Resistance in Humans>[pp. 4–6]{ref}
Back to article page
