= Isolation of the lac repressor by Walter Gilbert and Benno Müller-Hill
{tag=Work by Walter Gilbert}
{tag=Work by Benno Müller-Hill}
{title2=1966}
https://pubmed.ncbi.nlm.nih.gov/16591435/
Walter Gilbert and Benno Müller-Hill, Proceedings of the National Academy of Sciences USA 56(6), 1891–1898, December 1966. DOI 10.1073/pnas.56.6.1891. The original first-page byline and opening negative-control/operator-binding model are also indexed in the university-hosted scan: https://www.bio.davidson.edu/courses/synthetic/papers/Gibert_Muller_hill_66.pdf .
Additional exact primary locator: printed p. 1891, assay description and Design of the Experiment, in the already listed university-hosted original scan https://www.bio.davidson.edu/courses/synthetic/papers/Gibert_Muller_hill_66.pdf . The assay detects binding to inducer by equilibrium dialysis against radioactive IPTG, deliberately avoiding dependence on the exact repression mechanism. This is distinct from the opening operator-DNA-binding model and adds experimental specificity to the existing source card.
Back to article page
