= Klenow fragment
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The <Klenow fragment> is a large fragment of <Escherichia coli> <DNA polymerase I> that retains <DNA> polymerization and proofreading <exonuclease> activities while lacking the intact <enzyme>’s 5′-to-3′ <exonuclease> domain. This separation made it useful for extending <DNA> ends without all the activities of the full <enzyme>. Engineered exonuclease-deficient variants are different reagents, so a statement about retained proofreading should specify the form being discussed. <Molecular Biology of the Cell, 4th edition by Bruce Alberts and others>[Chapter 8, Manipulating Proteins, DNA, and RNA: DNA polymerases and end labelling]{ref}
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