NBD (source code)
= NBD
{c}
= Nitrobenzoxadiazole fluorophore
{synonym}
In <fluorescence> labeling, <NBD> denotes a nitrobenzoxadiazole fluorophore used in fluorescent derivatives, including labeled <lipids>. Its emission depends on local surroundings, so changing brightness can report an environmental change as well as a change in the number of labels.
For example, comparing a labeled <lipid> in aqueous solution and in a <lipid bilayer> requires accounting for different emission efficiencies. A fixed-illumination measurement of <fluorescence> alone is not an absolute concentration measurement unless that response is calibrated. https://www.thermofisher.cn/cn/en/home/references/molecular-probes-the-handbook/tables/amine-reactive-environment-sensitive-fluorophores.html[Thermo Fisher Molecular Probes Handbook, Table 1.13 note: NBD derivatives have solvent-dependent emission and quantum yields]{ref}
Back to article page
