= P-element vector
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A <P-element vector> is an engineered <DNA> carrier that uses <P element> recognition sequences to introduce cargo into chromosomes when the required <P-element transposase> is available. The mobile carrier and the enzyme source can be separate. Retaining the needed element ends does a different job from encoding the enzyme that acts on them.<P element in FlyBase>[Transformation vectors and separate transposase sources]{ref}
In the 1982 transformation study, a <P-element vector> carrying a wild-type <Drosophila melanogaster rosy> sequence produced germline transformants with inherited chromosomal copies and restored eye colour. The visible phenotype tested function of the transferred <gene>, while integration and transmission to descendants provided different evidence about its physical and hereditary fate.<Genetic transformation of Drosophila with transposable element vectors by G. M. Rubin and A. C. Spradling>[Abstract, ry1 carrier, chromosomal copies and phenotype]{ref}
For an invented inheritance calculation, assume one autosomal insertion, a <heterozygote> crossed to an insertion-free animal, and ordinary segregation without viability or transmission distortion. Half the offspring are expected to inherit the insertion. This fraction is conditional on the genetic model; it is not the percentage of injected embryos that will be transformed. A fluorescent or visible signal alone also does not establish a stable single-copy insertion, and an inserted cargo need not express identically at every genomic location.
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