= Solution
About 80% of full-length-expressing <biological cells> have two conspicuous nuclear foci, whereas roughly 90% of <biological cells> expressing the deletion <protein> have none. Both <proteins> show nuclear <fluorescence>, so the result is not explained simply by complete exclusion of the deletion <protein> from the nucleus. \b[The <DNA-binding domain> is needed for efficient focal accumulation at a small number of host sites.] A plausible explanation is recognition of repeated host <DNA> sequences resembling the viral binding sites; clustered repeats and homotypic contacts would concentrate the <protein> enough to create visible foci.
Two spots are compatible with one locus on each <homologous chromosome> in a diploid <biological cell>. They do not prove diploidy, identify a <chromosome>, or exclude unresolved sister loci and cell-cycle effects. An alternative is DNA-dependent recruitment through a host complex. Matching <protein> abundance and confirming stability of the deletion construct would make the domain comparison stronger. Punctate <fluorescence> alone does not demonstrate a liquid condensate or show that a focus identifies an <origin of replication>.
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