= Solution
\b[ToxB strongly reduces sedimentable wild-type <actin>, whereas act1-117 shows little additional reduction from its already low baseline.] Wild-type <actin> is mainly in the pellet without drug and mainly in the <supernatant> with drug. Using the approximate bar heights, the pelleted fraction changes from $740/(740+120)\approx0.86$ to $280/(280+820)\approx0.25$. Thus the drug suppresses <polymer> accumulation but does not eliminate the measured pellet entirely.
For act1-117, the pellet is only about one-third of the recovered signal even without drug, and remains near that fraction with drug: approximately $340/(340+570)\approx0.37$ and $360/(360+660)\approx0.35$. The mutant is therefore relatively resistant to the drug's further effect under this assay, but it is not a wild-type-quality polymerizer with an unchanged high <polymer> mass. These are approximate within-pair fractions; unequal total recoveries and loading limit precise quantitative comparison. <sedimentation> also requires confirmation that pellet material is filamentous rather than aggregated.
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