= Solution
\b[Efficient and timely budding requires the normal <actin> network, but some initial <polarized cell growth> remains possible when visible <actin filaments> are greatly depleted.] Around 40% of control <biological cells> have new buds by two hours, rising to nearly 60% by six hours. Drug-treated <biological cells> show almost no budding at two hours but roughly 15–20% at four to six hours, and the phase images confirm small buds despite loss of normal bright <actin> structures.
The result therefore supports a large delay and reduction, rather than an absolute block to every initiation event. Polarity cues or localized membrane-fusion machinery could remain spatially organized without normal long <actin cables>. The images and bud counts do not establish completion of a daughter-cell cycle, normal <cytokinesis> or indefinite proliferation. Residual <actin filaments> below detection remain an alternative contributor. This distinction between initial bud emergence and completion of growth was directly examined in a quiescence-exit study.<Polarized growth in the absence of F-actin in Saccharomyces cerevisiae exiting quiescence by Annelise Sahin and others>[Bud-emergence and cell-cycle-completion experiments]{ref}
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