Solution (source code)

= Solution

\b[Some <cell membrane> delivery and fusion must continue in the <biological cells> whose buds actually grow, but it need not use normal long-range cable transport.] <secretory vesicles> can move by <molecular diffusion> or other non-directed motion and be preferentially captured, tethered and fused where a surviving polarity cue concentrates the relevant <vesicle docking> machinery. Spatial selectivity can therefore reside in the destination rather than in directed travel over the entire route. Less efficient delivery is consistent with delayed budding and small buds.

Short residual <actin> tracks could also contribute; the experiment does not prove that every <actin filament> is absent. Neither bud shape nor <Con-A> staining directly measures vesicle trajectories or fusion events. Tracking <secretory vesicles> together with a membrane-fusion reporter and a polarity marker would distinguish directed runs from <molecular diffusion> followed by localized capture, with expression and imaging controls for reporter effects. Random arrival with selective <vesicle docking> is a published mechanistic proposal for this physiological context, rather than a demonstrated trajectory in the present images.<Polarized growth in the absence of F-actin in Saccharomyces cerevisiae exiting quiescence by Annelise Sahin and others>[Discussion: vesicle delivery and preferential docking during polarized growth]{ref}