= Solution
The recovered vesicle fractions contain bands at the expected positions for the supplied <SNARE proteins>. The <v-SNARE> preparation contains its principal band, and the <t-SNARE> preparation contains both <t-SNARE> and the <SNAP25> doublet. Thus \b[protein-containing vesicle fractions have been recovered], consistent with successful reconstitution.
The <SDS-PAGE> experiment does not establish the orientation of the <proteins>, their proportion facing outward, the <stoichiometry> in an individual vesicle, or <membrane fusion>. Nor does a stained band prove that all of its <protein> is correctly folded and active. Those are separate functional and <membrane topology> questions.
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