Solution
= Solution
\b[There is no evidence for substantial lipid mixing during the cold preincubation.] The trace begins near its low-fluorescence baseline when warming starts; a substantial pre-existing dilution of the dyes would already have raised that baseline. Nevertheless, <vesicle docking> or <trans-SNARE complex> formation can accumulate while the later membrane-rearrangement step remains slow. Absence of dequenching does not mean that the vesicles have had no interaction.