= Solution
\b[A single predicted monomer structure is insufficient.] It may suggest a possible binding pocket, domain hinge or subunit interface, and a confident multimer model may support a particular assembly hypothesis. Those features do not establish that alternative conformations are populated, that <ligand> binding shifts their populations, or that occupancy at one site changes another site's binding strength.
Cooperative <allostery> concerns free-energy relationships among states, not only one static geometry. Structural comparisons across conditions, independent oligomeric-mass measurements and ligand-dependent binding or activity curves are needed to test the proposal. A low-confidence inter-domain orientation is likewise uncertainty about a model, not a prediction that the <protein> is necessarily an allosteric oligomer.
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