Phase-contrast image artifacts
= Phase-contrast image artifacts
A finite <phase plate> also intercepts diffracted light, altering low <spatial frequencies>. Halos and shade-off can therefore mimic borders or obscure interiors.<Introduction to Phase Contrast Microscopy by Nikon Instruments Inc.>[Interpretation of Phase Contrast Images, halo and shade-off]{ref} In the <coherent zero-mode model of phase contrast>, the separation is perfect by assumption, so that model cannot predict those artifacts.