= Pohl Milón
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\b[Pohl Milón] is a researcher in <biophysics> and <biochemistry> whose work includes the timing of bacterial <translation initiation>. As first author of a 2012 kinetic study, he and collaborators investigated how <bacterial translation initiation factors> assemble on the <30S ribosomal subunit>.<Real-time assembly landscape of bacterial 30S translation initiation complex by Pohl Milón and others>[Byline and Abstract]{ref}
The study resolved a favored sequence in which <bacterial initiation factor IF2> and <bacterial initiation factor IF3> form an initially unstable assembly, then <bacterial initiation factor IF1> stabilizes it. <Messenger RNA> can arrive at different stages, depending on its concentration and structure. Thus a final composition does not specify one compulsory order of assembly.<Real-time assembly landscape of bacterial 30S translation initiation complex by Pohl Milón and others>[Abstract and Figures2–4,6–7, assembly kinetics and mRNA recruitment]{ref}
An original waiting-time model shows the value of kinetic measurements. If two obligatory sequential steps have independent <exponential distributions> with rates $10\,\mathrm{s^{-1}}$ and $2\,\mathrm{s^{-1}}$, their mean waiting times add to $0.10+0.50=0.60$ seconds. Doubling the faster rate reduces this mean to 0.55 seconds; doubling the slower rate reduces it to 0.35 seconds. These stipulated rates are not experimental fits. They show why identifying the slow step can matter more than simply counting how many components bind.
\Image[https://biotune.upc.edu/en/shared/personal/pohl_milon.jpg/@@images/image-0-4f4d822dd202aecfed5533faba841b2b.jpeg]
{title=Pohl Milón, named portrait in the Bio-TUNE consortium's Universidad Peruana de Ciencias Aplicadas partner profile}
{height=300}
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