Phosphorimager Created 2026-10-14 Updated 2026-11-05
A phosphorimager reads stored radiation exposure from a reusable phosphor screen to form a digital image. In molecular experiments, spatial signal can quantify radiolabel distributed across a gel or blot. Background, saturation, exposure and detector response affect the inferred amount of labelled material. A band intensity therefore requires calibration and normalization before being interpreted as a molecule count or reaction rate. [ref]