= Real-time polymerase chain reaction
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= Quantitative PCR
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= qPCR
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<Real-time polymerase chain reaction> monitors <fluorescence> during cycling. With validated specificity, calibration and <PCR efficiency>, that record supports estimating initial template amount; this quantitative use is called <qPCR>. Ordinary endpoint <PCR amplification> examines accumulated product afterwards. <qPCR> need not start from <RNA>, and <RT-PCR> need not monitor <fluorescence>. Combining both operations gives <RT-qPCR>.<Real-Time PCR (qPCR) Basics by Thermo Fisher Scientific>[Real-Time PCR Basics: amplification monitoring and quantification]{ref}
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