Renilla luciferase
= Renilla luciferase
{c}
A luminescent reporter <enzyme> often used as a separately measured reference in a dual-luciferase assay. Valid normalization requires a reference insensitive to the experimental manipulation.
Its reaction oxidizes coelenterazine using <oxygen> and differs chemically from the ATP-dependent firefly system. Using a distinct reaction permits separate optical readouts, but <substrate> availability and <enzyme> behaviour still need to be considered when comparing signals. <Enzyme Nomenclature by the IUBMB>[EC 1.13.12.5, reaction and comments]{ref}