= Reverse transcription polymerase chain reaction
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= RT-PCR
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<RT-PCR> combines <reverse transcription> with <PCR amplification>. The RT names the <RNA>-to-<DNA> step, not real-time observation. <RT-PCR> can have an endpoint readout. A matched preparation omitting <reverse transcriptase> tests whether contaminating <DNA> could account for amplification; it answers a different question from a no-template reaction.<Basic Principles of RT-qPCR by Thermo Fisher Scientific>[One-step vs. Two-step RT-qPCR and Controls for RT-qPCR: minus-reverse-transcription control]{ref}
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