= RNA-peptide fusions for the in vitro selection of peptides and proteins by Richard W. Roberts and Jack W. Szostak
{c}
{tag=Work by Richard W. Roberts}
{tag=Work by Jack W. Szostak}
{title2=1997}
https://pmc.ncbi.nlm.nih.gov/articles/PMC24913/
Proceedings of the National Academy of Sciences USA 94(23), 12297–12302 (11 November 1997), DOI 10.1073/pnas.94.23.12297. Exact full first bylines Richard W Roberts and Jack W Szostak. Abstract and introductory rationale describe puromycin-mediated RNA-peptide fusions linking a peptide to its encoding mRNA; immunoprecipitation enriched an encoded myc epitope peptide from random fusion material.
Additional exact locator: Selective Enrichment of an RNA-Peptide Fusion and Figure 5, https://pmc.ncbi.nlm.nih.gov/articles/PMC24913/ . Before/after anti-myc immunoprecipitation aliquots were reverse-transcribed and PCR-amplified with a radiolabeled primer; restriction digestion distinguished the myc encoding sequence from the pool. The figure also notes preferential translation/reverse transcription, so recovered sequence ratios are not automatically unbiased input ratios.
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