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Joined 2026-09-21 Message
Incoming links: Saurabh Upadhyay
An Optimized Enzyme-Coupled Spectrophotometric Method for Measuring Pyruvate Kinase Kinetics by Saurabh Upadhyay Created 2026-10-18 Updated 2026-10-31
By Saurabh Upadhyay. Published in Bio-protocol 15(16), e5415, on 20 August 2025; available online 5 August 2025. Bio-protocol publishes and hosts the protocol; the author's Indian Institute of Technology, New Delhi affiliation is distinct from authorship. PMC is an archival mirror.
Online text: en.bio-protocol.org/en/bpdetail?id=5415&type=0
Cited passages: graphical overview caption; Equipment item 1; Procedure A.5–6 and B.2.a–i; Figure 2 caption; Data analysis §3; Validation of protocol; General notes items 4 and 6; PDF printed pp. 7–10. Procedure B.2.i and Data analysis §3 give different optical path lengths; no absolute reported rate is adopted.
Saurabh Upadhyay Created 2026-10-18 Updated 2026-11-05
Saurabh Upadhyay authored an enzyme-coupled spectrophotometric method for measuring pyruvate kinase kinetics. A coupled reaction converts product formation into a change in an optical reporter.
The reporter measures the intended enzyme rate only when coupling is sufficiently rapid and the signal is converted with an appropriate optical path length. This separates an observed absorbance slope from an absolute chemical rate.[ref]
An Optimized Enzyme-Coupled Spectrophotometric Method for Measuring Pyruvate Kinase Kinetics by Saurabh Upadhyay. Graphical overview and Data analysis section 3
Identity source: pubmed.ncbi.nlm.nih.gov/40873479/