SEC chromatogram
= SEC chromatogram
{c}
A <SEC chromatogram> plots a detector signal against elapsed time or elution <volume (thermodynamics)>. At constant <volumetric flow rate> $Q$, the volume delivered after injection is $Q(t-t_{\rm injection})$; subtraction of extra-column transport volume is needed for a column-only $V_e$. Real peak widths include injection volume, column dispersion, and tubing/detector spreading. Peak position, width, and area answer different questions.