Sec13-Sec31 complex
= Sec13-Sec31 complex
{c}
The <Sec13-Sec31 complex> forms the outer layer of a <COPII> coat. Its assemblies provide a scaffold around a budding carrier, while the inner <Sec23-Sec24 complex> couples coat formation to <Sar1 GTPase> and cargo selection. Reconstitution using purified coat components and defined <liposomes> demonstrated that this machinery can generate coated carriers without the full complexity of a <cell>. <COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes by Ken Matsuoka and others>[1998, purified Sar1p, Sec23-24p and Sec13-31p reconstitution]{ref}