= Sequencing depth
<Sequencing depth> describes the amount of sequence observation obtained, but its exact convention depends on the assay. For a <genome>, nominal mean depth can be approximated by total sequenced bases divided by target length; in <RNA sequencing>, depth often means the number of reads generated. Larger depth improves sampling of rare material but does not fix systematic bias or substitute for biological replication. <Talking Glossary of Genomic and Genetic Terms by NHGRI>[Coverage entry]{ref}
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