= Single-particle tracking PALM
= sptPALM
{c}
{synonym}
<Sparse photoactivation> allows successive positions of individual labelled molecules to be linked into trajectories; activation of fresh subsets yields many tracks in living cells. This extends <photoactivated localization microscopy> from accumulated positions to mobility. For free two-dimensional <diffusion> with independent per-coordinate <fluorescence localization precision> $\sigma$, instantaneous frames give mean squared displacement $4D\Delta t+4\sigma^2$. <Motion blur>, tracking errors, confinement and photobleaching must be accounted for before assigning mobility classes.<High-density mapping of single-molecule trajectories with photoactivated localization microscopy by Suliana Manley and others>[Abstract and pages 155–156: combining sparse activation with single-particle trajectories]{ref}
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