= Stochastic optical reconstruction microscopy
= STORM
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<Stochastic optical reconstruction microscopy> reconstructs an image from the estimated positions of individual fluorescent labels that are observed in sparse subsets over many frames. The experiment repeatedly switches labels between detectable and dark states, fits isolated <point spread functions>, and combines the localizations.
This separates emitters in time instead of requiring all neighboring labels to be spatially resolved in one exposure. <Localization precision> depends on detected <photons> and background; drift, incomplete labeling and simultaneous overlapping emitters constrain final <optical resolution>. In the original demonstration, reversibly switched dye labels enabled repeated sparse localization.<Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) by Michael J. Rust and others>[Abstract and opening results: reversible photoswitching and reconstruction from individual localizations]{ref}
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