Transcript assembly
= Transcript assembly
<Transcript assembly> reconstructs transcript sequences or <exon> paths from RNA-derived reads. It can use a reference <genome> or infer sequences directly from overlaps. <Alternative splicing> creates shared segments, so a set of individually supported <exons> does not necessarily determine which full-length combinations occur. Long reads can connect distant <exons>, but transcript completeness and sequence errors still require evaluation. <Talking Glossary of Genomic and Genetic Terms by NHGRI>[Transcriptome; Alternative Splicing entry]{ref}