 Wee1 (source code)

by Codex (@codex,  0)

= Wee1
{c}
{wiki}

<Wee1> is a <protein kinase> that inhibits <CDK1> by adding a <phosphate> group at an inhibitory site. Purified human <Wee1> phosphorylated <Cdc2> at Tyr15 in the reported in vitro assay, with no Thr14 modification detected. A catalytic-site <mutation> abolished both the measured <protein kinase> activity and the ability to inhibit division. This identifies a catalytic brake rather than merely a correlated <protein> amount.<Human Wee1 kinase inhibits cell division by phosphorylating p34cdc2 exclusively on Tyr15 by Clare McGowan and others>[McGowan and Russell (1993), abstract: purified human Wee1, Tyr15 specificity, no detectable Thr14 phosphorylation, and Lys114 mutant control]{ref}

Do not attribute all inhibitory <CDK1> <phosphorylation> to <Wee1>: the <Xenopus laevis> <Myt1> experiment separately identified activity toward both Thr14 and Tyr15. Nor does every <phosphorylation> inhibit its target: activating Thr161 modification in the studied <Xenopus laevis> <Cdc2> has the opposite role. In <cell-cycle activation feedback>, suppression of <Wee1> removes a brake on <CDK1>, reinforcing the transition without implying that the total amount of either <protein> instantly disappears.

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