= Western blot loading control
A <Western blot loading control> estimates lane-to-lane sample differences. A reference <protein>, such as <actin>, must have stable abundance and detection responsive to amount. Equal saturated bands fail to establish equal loading. <Considerations when quantitating protein abundance by immunoblot by Alicia A. McDonough and others>[McDonough and others, “Consider the Loading Controls”, Figure 4: sample doubling changes claudin detection but not the actin signal]{ref}
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