A simple and very efficient method for generating cDNA libraries by U. Gubler and B. J. Hoffman 2026-11-05
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U. Gubler and B. J. Hoffman, Gene 25(2–3), 263–269, November 1983. DOI 10.1016/0378-1119(83)90230-5. The abstract describes first-strand synthesis followed by RNase H/DNA-polymerase-I second-strand synthesis, cloning from submicrogram mRNA amounts, and sequencing of a known bovine preproenkephalin transcript to test full-length fidelity.
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