Coupled 5′ nucleotide recognition and processivity in Xrn1-mediated mRNA decay by Martin Jinek and others 2026-11-05
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Martin Jinek, Scott M. Coyle and Jennifer A. Doudna; Molecular Cell41(5),600–608(2011), DOI10.1016/j.molcel.2011.02.004. Primary indexed full text and publisher PDF www.cell.com/article/S1097276511000876/pdf verified. Results/Figure3: phosphate-pocket and His41 mutations, radiolabeled23-mer RNA degradation on denaturing gel/phosphorimaging. Figure4: hydrolysis-coupled unwinding of defined RNA/DNA hybrids with2-,5-,8-nt RNA overhangs. Structure is a truncated D207A Drosophila construct complexed with11-mer DNA at3.2Å, not an unmodified full-length RNA complex. Direct PMC landing page challenged.
Additional locator: Figure 3C shows cleavage of a 5′-OH substrate under the tested single-turnover conditions. The reported preference for a 5′ phosphate is not an unconditional prohibition of activity on every other substrate.
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Jennifer A. Doudna faculty profile at Berkeley 2026-11-05
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Verified locator: Named Jennifer A. Doudna institutional profile and Selected Publications explicitly lists the 2012 programmable dual-RNA paper. Named portrait mcb.berkeley.edu/sites/default/files/images/faculty/doudnaj_250_309.jpg , photo credit Keegan Houser.
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