Stabilization of RNA during laser capture microdissection by performing experiments under argon atmosphere or using ethanol as a solvent in staining solutions by Mathieu Clément-Ziza and others 2026-11-05
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Mathieu Clément-Ziza and colleagues, RNA 14(12), 2698–2704 (2008); DOI 10.1261/rna.1261708. Primary abstract and Figure 1: argon atmosphere or ethanolic fixation/staining stabilized RNA during the tested microdissection period, up to 90 minutes. Figure 1 compares RNA integrity measurements at multiple times; Figure 2 shows selected human-colon ganglia and neurons before/after collection. The observed RNA preservation does not by itself establish unchanged living-cell transcription. Full first-author spelling follows the primary indexed byline.
Additional precise Figure 1 caption locator: RNA extracted from section scrapes on a slide was assessed at different times after staining; experiments were repeated at least three times and quality was evaluated on an Agilent Bioanalyzer with the RIN algorithm. Panels include preparation comparisons and material collected after 30, 60 and 90 minutes. This identifies the instrumental/replicate design separately from the already recorded up-to-90-minute preservation conclusion. pubmed.ncbi.nlm.nih.gov/18945804/
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