Journal of Biological Chemistry286(10),7727–7731(2011), DOI10.1074/jbc.C111.219022. Full bylines Naoko Tanaka and Stewart Shuman verified. Abstract and Figure1: purified E. coli RtcB seals a broken tRNA-like stem-loop bearing cyclic-phosphate/5'-OH ends; labelled products analyzed by denaturing PAGE. In-vitro joining demonstrated; broad physiological repair roles were proposed, not all proven by this experiment.