Basic Principles of RT-qPCR by Thermo Fisher Scientific Created 2026-10-14 Updated 2026-10-31
Publisher: Thermo Fisher Scientific.
This source is credited to an organization.
Cited passages: One-step vs. Two-step RT-qPCR and Controls for RT-qPCR: minus-reverse-transcription control; RNA as the Starting Material and Reverse Transcriptase Enzymes.
PCR Cycling Parameters—Six Key Considerations for Success by Thermo Fisher Scientific Created 2026-10-14 Updated 2026-10-31
Publisher: Thermo Fisher Scientific.
This source is credited to an organization.
Cited passages: PCR cycle number determination and Figure 7: lowered efficiency and plateau from reaction-component depletion and by-products.
Additional cited passages: Template DNA denaturation assessment, paragraphs 1 and 3–6; DNA denaturation considerations, paragraph 1; Primer annealing optimization, opening, calculated-setting and nonspecific-product paragraphs; Primer extension considerations, paragraphs 1 and 3. No human byline or publication or revision date was verified.
Principles of PCR by Thermo Fisher Scientific Created 2026-10-14 Updated 2026-10-31
Publisher: Thermo Fisher Scientific.
This source is credited to an organization.
Cited passages: Principles of PCR, accompanying description: denaturation, annealing, primer-dependent extension and dNTP incorporation.
Additional cited passages: accompanying Description, first three paragraphs, strand separation, complementary primer binding, enzyme-dependent extension temperature, dNTP incorporation and two-step cycling. Accompanying text was inspected; frame-by-frame video viewing is not asserted. No human byline or publication or revision date was verified.
Real-Time PCR (qPCR) Basics by Thermo Fisher Scientific Created 2026-10-14 Updated 2026-10-31
Publisher: Thermo Fisher Scientific.
This source is credited to an organization.
Cited passages: Real-Time PCR Basics: amplification monitoring and quantification.
Official institutional website. Official instrument technical documentation. Webpage publication date unestablished.