ImageJ User Guide by Tiago Ferreira and Wayne Rasband 2026-11-05
View more
Title page verifies both author names and version1.46r, last updated2October2012. Section8, Stacks, Virtual Stacks and Hyperstacks: ordered spatial/time images, equal dimensions and bit depth, multiple coordinate axes and disk-backed stacks. Only computing/data representation discussed.
Additional IJ1.46r locators: §28.4, Image Properties, spatial spacing, voxel depth, frame interval and pixel-unit origin; §§30.1 and30.7, Measure and Set Measurements; §§30.8–30.9, Set Scale and intensity Calibrate; Global Calibrations, numbered note24.
Additional IJ1.46r locators: §19, area, line and point selection tools; §30.14.5, ROI Manager, selection persistence and slice associations; §30.7, Area measurement; §30.8, Set Scale.
Further IJ1.46r locators: §27.12.10, Create Mask, area selection encoded as255 inside and0 outside, with an inverting display lookup table unless Black Background is checked; §27.12.9, Make Inverse, complementing a selection.
Further IJ1.46r locators: §§28.15.1–28.15.2, Invert LUT changes display mapping, whereas Apply LUT changes pixel values and restores an identity table; §27.11, Invert changes pixel values; §27.12.10, Create Mask and its display lookup table.
Read the full article