Yaodong Yang (source code)

= Yaodong Yang
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<Yaodong Yang> is the first author of a 2006 study directly characterizing the <auxin> influx carrier <AUX1>. The team expressed this <plant> <membrane transport> <protein> in <Xenopus> <oocytes> and measured entry of radioactively labeled <indole-3-acetic acid>. Uptake was saturable and depended on <pH>, providing functional evidence about transport rather than only the <protein>'s location.<High-affinity auxin transport by the AUX1 influx carrier protein by Yaodong Yang and others>[Abstract: heterologous expression and radiolabeled IAA uptake; Current Biology 16, 1123–1127, 2006]{ref}

The mutant comparison connected molecular and organismal observations. Changes in <AUX1> that disrupted <auxin> responses in <plants> also reduced uptake in the experimental <oocytes>. Testing the same altered <protein> in a different cellular setting helped distinguish its transport activity from the many downstream effects of <auxin> on <plant development>. Saturation also indicates a limited transport capacity under the assay conditions, rather than an uptake rate that increases without bound.<High-affinity auxin transport by the AUX1 influx carrier protein by Yaodong Yang and others>[Abstract: mutant uptake, concentration dependence and physiological relevance]{ref}