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For the negative-sign equation, take a nonzero separated solution $\varphi =X(x)Y(y)$. Separation of variables gives $X^{\prime \prime }=-\mu X$ and $Y^{\prime \prime }=-(25-\mu )Y$. The zero Dirichlet boundary conditions on $X$ force $\mu =m^2$ with a positive integer $m$ and $X=\sin mx$, up to a constant. Zero boundary values on $Y$ similarly require $25-m^2=n^2$ with a positive integer $n$. A zero or negative separation eigenvalue would give linear or hyperbolic factors, whose two zero endpoint values force the trivial solution.

The only positive integer pairs with $m^2+n^2=25$ are $(3,4)$ and $(4,3)$. By the complete Dirichlet eigensystem of a rectangle, these span the whole eigenspace, soFor the positive-sign equation there is only the zero solution. One can use the same strictly positive eigenvalues of $-\Delta$, or directly apply the zero Dirichlet solution of the modified Helmholtz equation. Multiplying by $\varphi$ and using Green's first identity giveswhere $\Omega =(0,\pi {)}^2$. Both nonnegative integrals must vanish, proving $\boxed{\varphi =0}$.